cefoxitin sodium salt  (Thermo Fisher)

Journal: iScience

Article Title: The l,d -transpeptidation pathway is inhibited by antibiotics of the β-lactam class in Clostridioides difficile

doi: 10.1016/j.isci.2025.112227

Figure Lengend Snippet: Impact of different β-lactam antibiotics on the PG structure of vegetative cells of C. difficile wild type and ΔΔΔ ldt (A) LC-MS chromatogram of muropeptides from vegetative cells of C. difficile wild-type (WT) strain grown in the presence of subinhibitory concentrations of cefoxitin. Peak labels refer to Table S1 . New major peaks observed in the presence of the antibiotics are labeled with letters. See also Table S1 for the structure of all identified muropeptides. Data are representative of three independent experiments. (B) Abundance of muropeptides with a tetrapeptide or a pentapeptide stem (free side or acceptor chain in multimers) in the PG of C. difficile wild-type and ΔΔΔ ldt strains grown in the absence of antibiotics (no ATB) or in the presence of cefoxitin or meropenem. (C) Abundance of muropeptide dimers with a 4→3 and a 3→3 cross-link relative to total muropeptides in the PG of C. difficile wild-type and ΔΔΔ ldt strains grown in the absence of antibiotics (no ATB) or in the presence of cefoxitin or meropenem. (D) Cross-linking index of the PG of C. difficile wild-type and ΔΔΔ ldt strains grown in the absence of antibiotics (no ATB) or in the presence of cefoxitin or meropenem. (E) Abundance of muropeptide dimers with a 3→3 cross-link relative to the total cross-links in dimers in the PG of C. difficile wild-type and ΔΔΔ ldt strains grown in the absence of antibiotics (no ATB) or in the presence of cefoxitin or meropenem. (F) Abundance of muropeptides with a pentapeptide stem ending with a non-canonical d -amino-acid (NCDAA: Gly, Phe, Leu, or Val) (Penta-NCDAA ) relative to the total pentapeptide muropeptides in the C. difficile wild-type and ΔΔΔ ldt strains grown in the presence of cefoxitin or meropenem. All graphs represent mean ± SEM and include individual data points; n = 3 independent experiments. ∗∗∗ p ≤ 0.01 and ∗∗∗∗ p ≤ 0.001 by a two-way ANOVA followed by a Dunnett’s multiple comparisons test.

Article Snippet: Cefoxitin sodium salt , Thermo Scientific , 455280050.

Techniques: Liquid Chromatography with Mass Spectroscopy, Labeling

Journal: iScience

Article Title: The l,d -transpeptidation pathway is inhibited by antibiotics of the β-lactam class in Clostridioides difficile

doi: 10.1016/j.isci.2025.112227

Figure Lengend Snippet:

Article Snippet: Cefoxitin sodium salt , Thermo Scientific , 455280050.

Techniques: Recombinant, Sequencing, Software, Targeted Proteomics

Journal: mSphere

Article Title: Metabolic changes associated with polysaccharide utilization reduce susceptibility to some β-lactams in Bacteroides thetaiotaomicron

doi: 10.1128/msphere.00103-24

Figure Lengend Snippet: Carbon source modulates the susceptibility of Bth to β-lactams independent of β-lactamase activity. ( A–C ) Percent growth plot for AMX treatment of Bth grown in MM with different carbon sources ( A ), different concentrations of glucose ( B ), and different concentrations of glucose and pectin ( C ). Data are represented as the average percent growth compared with untreated control cultures ± SEM. G = glucose, P = pectin. ( D ) Percent growth plot for Bth grown in MM-glucose or MM-pectin and treated with various β-lactams. Data are represented as the average percent growth compared with untreated control cultures ± SEM. G = glucose, P = pectin, AMX = amoxicillin, AMP = ampicillin, FOX = cefoxitin, FEP = cefepime, CRO = ceftriaxone, MEM = meropenem. ( E and F ) Percent growth plot for AMX plus tazobactam treatment of Bth grown in MM-glucose ( E ) or MM-pectin ( F ). Data are represented as the average percent growth compared with untreated control cultures.

Article Snippet: Amoxicillin, ampicillin sodium salt, cefoxitin sodium, ceftriaxone sodium, cefepime hydrochloride monohydrate, or meropenem trihydrate (purchased through Thermo Fisher or Sigma-Aldrich) were added at varying concentrations to cell culture media and serially diluted twofold.

Techniques: Activity Assay, Control

Journal: BioMed Research International

Article Title: A Promising Approach to Provide Appropriate Colon Target Drug Delivery Systems of Vancomycin HCL: Pharmaceutical and Microbiological Studies

doi: 10.1155/2014/182197

Figure Lengend Snippet: Biostatic activity to different antibiotics against S11, S18, and S29 isolates.

Article Snippet: MIC of oxacillin (oxacillin sodium monohydrate, Sigma-Aldrich, St. Louis, MO, USA), cefoxitin (cefoxitin sodium salt, Sigma-Aldrich, St. Louis, MO, USA), and vancomycin (vancomycin hydrochloride, Mylan, Morgantown, USA) was determined by agar dilution method as described elsewhere [ ].

Techniques: Activity Assay